ASTM E2149 PDF

ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.

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ASTM E – Microbe Investigations (MIS)

Link to Active This link will always route to the current Active version of the standard. All the samples are shaken in dynamic shake flasks in a wrist action shaker to ensure good contact between the bacteria and the treated fibre, fabric, or other substrate by constant agitation of the test specimen in a bacterial suspension during the test period. It has been developed for routine quality control and screening tests and is used to evaluate substrate bound antimicrobials.

The presence of a leaching antimicrobial is determined post-test. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Microbial concentrations in the treated and untreated samples are usually determined at 0, 1 or 24 hours contact time.

A single cookie will be used in your browser to remember your preference not to be astj. Following exposure, a sample of the aetm organism suspension is removed quantitatively assayed for survivors. Work Item s – proposed revisions of this standard. In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli.

This method is generally used to substantiate antimicrobial properties of treated specimens for non-public health related claims. The flask is placed onto a wrist-action shaker and shaken for a desired exposure time, typically 1 hour. These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use 2e149 inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.

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This standard does not purport to address all of the safety concerns, if any, associated with its use. These cookies collect information about how you interact with our website and allow us to remember you.

It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Materials evaluated by this method may be comprised of fabric, paper, powder or other solid materials. To determine if a compound is immobilized in all conditions or during the end use of the product additional testing may be required.

Link to Active This link will always route to the current Active version of the standard.

ASTM E – Method Determining the Antimicrobial Activity

This standard does not purport to address all of the safety concerns, if any, associated with its use. Organisms typically used in this method: This test ensures good contact between bacteria and the treated fibre by constant agitation of the test specimen in a bacterial suspension during the test qstm 1hour.

Historical Version s – view previous versions of standard. No regulated reduction limits currently exist for general antimicrobial claims made using this method. The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time. To find out more about the cookies we use, see our Privacy Policy.

Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard. The number of viable organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls. Surface antimicrobial activity is determined by comparing results from the test sample to controls run simultaneously.

Record and report presence of solution activity.

ASTM E2149 Antimicrobial Activity

Summary of Test In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli. Accept Decline Privacy policy. These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.

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If an antimicrobial agent that aastm shown to be removed from the surface by Section 13 is utilized in this test methodology, controls must be included such that appropriate neutralization steps are including during recovery and enumeration. The antimicrobial activity of a substrate-bound, non-leaching antimicrobial agent is dependent upon direct contact of microbes with the active chemical agent.

Work Item s – proposed revisions of this standard. This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism. The susceptibility of the species to particular biocides could be altered depending on its life stage cycle. Facebook Linkedin Instagram Twitter Google.

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The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension. We use this information to improve and customize your browsing experience and for analytics and metrics about our visitors — both on this website and other media. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.

This website stores cookies on your computer. Test Package Request Form. No other units of measurement are included e21449 this standard. Log in Log out Edit.

Summary of Test Method: