Category, Microbiology Laboratory. Test, Test Method Description. Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. TEST METHODS ARE DELIVERED AS A LINK IN YOUR EMAILED RECEIPT. AATCC TM, Antibacterial Activity Assessment of Textile Materials. Current individual AATCC test methods and other standards for textiles are Fabrics, Assessment of Textile Materials: Parallel Streak Method, Test Method
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AATCC Test Method
Parallel Streak Method Developed in by AATCC Committee RA31; reaffirmed, ; editorially revised,; revisedwith title change; editorially revised and reaffirmed Wear safety glasses in all laboratory areas.
Other organisms can also be used depending on the metohd end-use. Plug and sterilize at kPa 15 psi for 15 minutes. To constitute acceptable antibacterial activity, there must be no bacterial colonies directly under the sample in the aatcx area. The Parallel Streak Method has proven effective over a number of years of use in providing evidence of antibacterial activity against both Gram positive and Gram negative bacteria.
The average width of a zone of inhibition along a streak on either side text the test specimen may be calculated using the following equation: Your comments are invited either for revision of this test method or for additional methods and should be addressed to the AATCC Technical Center. Antibacterial Activity Assessment of Textile Materials: A standard strain of bacteria is used which aaycc specific to the requirements of the material under test.
Users of this test method are expressly advised that determination of the validity of any such aarcc rights, and the risk of infringement of such rights, are entirely their own responsibility. If you feel that your comments have not received a fair hearing, you should make your views known to Christopher S. Maintenance of Culture of Test Organisms 8.
Culture Medium mm length permits the specimens to lie across 5 parallel inoculum axtcc each of diminishing width from about 8 mm to 4 mm wide. Rectangular specimens cut 25 – 50 mm They etst be any convenient size. Treated materials should be compared to an untreated corresponding material and a material specimen with known bacteriostatic activity.
Other recommended strains are listed below in Section 6. Individual reprints single or multiple copies of this method may be obtained by contacting AATCC at the above address or tel: Therefore, every necessary and 5.
Specimens of the test material, including corresponding untreated controls of the same material, are placed in intimate contact with growth agar which has been previously streaked with test organism. Precision and Bias 7. Allow agar to gel firmly before inoculating. Department of Health and Human services publication Biosafety in Microbiological and Biomedical Laboratories should be consulted see Leonard, Technical Ttest, at the emthod shown below.
Peptone Bacto-peptone see Avoid contamination by using good sterile technique in plating and transferring.
Some of the bacteria used in this test are pathogenic; i. Mix well using appropriate agitation. All OSHA standards and rules must also be consulted and followed.
Therefore, when the intent is to demonstrate bacteriostatic activity by the diffusion of the antibacterial agent through agar, Method fulfills this need. Check culture purity by making streak plates periodically and observing for a single species-characteristic type of colonies.
Your comments will receive careful consideration at a meeting of the responsible technical committee, which you may attend.
Antimicrobial – Parallel Streak Method AATCC 147
The test is metyod in the cases where the antibacterial agent is migratory i. In addition, the electronic file may not be distributed elsewhere over computer networks or otherwise. If no other bacterial species is specified, Staphylococcus aureus may be used as a representative Gram positive organism. These safety precautions are for information purposes only.
AATCC Antimicrobial Testing Lab – Accugen Laboratories, Inc.
In the Parallel Streak Method, the agar surface is inoculated making it easier to atcc between the test organism and contaminant organisms which may be present on the unsterilized specimen. Safety Precautions reasonable precaution must be taken to eliminate this risk to the laboratory personnel and to personnel in the associated environment.
The size of the zone of inhibition and the narrowing of the streaks caused by the presence of the antibacterial agent permit an estimate of the residual antibacterial activity after multiple washings.
Neither the electronic file nor the hard copy print may be reproduced in any way.
At the conclusion of two weeks, make a fresh transplant from stock culture. Foreword The Parallel Streak Method has filled a need for a relatively quick and easily executed qualitative method to determine antibacterial activity of diffusable antimicrobial agents on treated textile materials. This is not necessary if prepared, dehydrated medium is used.
AATCC MethodAntibacterial Finishes mmethod Textile Materials, Assessment of, is a quantitative procedure which is adequately sensitive but methhod cumbersome and time consuming for routine quality control and screening tests. The results of using this procedure have been demonstrated by Committee RA31 to be reproducible by various laboratories working with materials containing residual amounts of antibacterial agents as determined by chemical assay after multiple standard washings.
This test method is subject to revision at any time by the responsible technical committee and must be reviewed every five years and if not revised, either reaffirmed or withdrawn.
The method is useful for obtaining a rough estimate of activity in that the growth of the inoculum organism decreases from one end of each streak to the other and from one streak to the next resulting in increasing mrthod of sensitivity.
AATCC grants you a license as follows: This may be accomplished more easily by pressing the specimen to the agar surface with a biological section lifter or with a spatula which has been sterilized by flaming and then air cooled immediately before use.
Notes text References Adjust to pH 6. Uses and Limitations 1. May be sterilized in 1, mL borosilicate glass flasks and petri dishes poured from this.